Journal: Oncology reports

Article Title: PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway.

doi: 10.3892/or.2015.4096

Figure Lengend Snippet: Figure 1. Expression of PIG3 in PTC and normal thyroid tissues determined by immunohistochemistry. The PIG3 expression in normal follicular cells is noted by the white arrow (A,A'). PTC showing strong PIG3 expression (black arrow) (B, B') (Magnification, x100 and 400 for A and B, and A' and B'). PTC, papillary thyroid carcinoma.

Article Snippet: The slides were then incubated overnight with primary rabbit polyclonal anti-PIG3 antibody (TA308561; OriGene, Rockville, MD, USA) at a dilution of 1:800 at 4 ̊C in a humid chamber.

Techniques: Expressing, Immunohistochemistry

Journal: Oncology reports

Article Title: PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway.

doi: 10.3892/or.2015.4096

Figure Lengend Snippet: Figure 2. Expression of PIG3 and P53 in PTC (*P<0.05). (A) The relative mRNA levels of PIG3 and p53 in normal thyroid tissue and PTC were determined by qPCR, with GAPDH as a control. (B and C) PIG3 and p53 protein expression was detected in PTC and normal thyroid tissue using western blot analysis. Each bar represents the mean of three independent.

Article Snippet: The slides were then incubated overnight with primary rabbit polyclonal anti-PIG3 antibody (TA308561; OriGene, Rockville, MD, USA) at a dilution of 1:800 at 4 ̊C in a humid chamber.

Techniques: Expressing, Control, Western Blot

Journal: Oncology reports

Article Title: PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway.

doi: 10.3892/or.2015.4096

Figure Lengend Snippet: Figure 3. Downregulation of PIG3 in PTC cells and effects of PTC on the colony formation and cell proliferation of CGTHW-3 and K1 cells (*P<0.05). (A) PIG3 mRNA levels were analyzed by qPCR. PTC cells were transfected with siRNA for PIG3 (siRNA) and NC, the untreated group (MOCK) with GAPDH as a control. (B and C) PIG3 protein levels in the three groups were analyzed using western blot analysis. GAPDH was used as an internal control. (D) CCK-8 cell proliferation assay for siRNA and NC‑transfected PTC cells. (E and F) Representative colony formation assay, the numbers of colonies in NC were set as 1. Each bar shows the mean of three independent experiments. PTC, papillary thyroid carcinoma; NC, negative control.

Article Snippet: The slides were then incubated overnight with primary rabbit polyclonal anti-PIG3 antibody (TA308561; OriGene, Rockville, MD, USA) at a dilution of 1:800 at 4 ̊C in a humid chamber.

Techniques: Transfection, Control, Western Blot, CCK-8 Assay, Proliferation Assay, Colony Assay, Negative Control

Journal: Oncology reports

Article Title: PIG3 plays an oncogenic role in papillary thyroid cancer by activating the PI3K/AKT/PTEN pathway.

doi: 10.3892/or.2015.4096

Figure Lengend Snippet: Figure 4. Knockdown of PIG3 modulation of PI3K/AKT/PTEN pathway activity in PTC cells (*P<0.05). (A and C) PI3K p110a, AKT, P-AKT and PTEN protein levels in the 2 groups were analyzed by western blot analysis after transfection with siRNA or NC in CGTHW-3 and K1 cells. Each bar represents the mean value and error information. NC, negative control.

Article Snippet: The slides were then incubated overnight with primary rabbit polyclonal anti-PIG3 antibody (TA308561; OriGene, Rockville, MD, USA) at a dilution of 1:800 at 4 ̊C in a humid chamber.

Techniques: Knockdown, Activity Assay, Western Blot, Transfection, Negative Control

Journal: CNS neuroscience & therapeutics

Article Title: Oral 7,8-Dihydroxyflavone Protects Retinal Ganglion Cells by Modulating the Gut-Retina Axis and Inhibiting Ferroptosis via the Indoleacrylic Acid-AhR-ALDH1A3-FSP1 Pathway.

doi: 10.1111/cns.70442

Figure Lengend Snippet: FIGURE 5 | IDA inhibits ferroptosis in retinal neurons via the AhR-ALDH1A3-FSP1 pathway. (a) Representative western blot images of f AhR-ALDH1A3-FSP1 pathway proteins. (b–d) Quantification of nAhR, ALDH1A3, and FSP1 levels relative to β-actin. IDA increased nAhR and ALDH1A3 levels, effects partially reversed by the AhR inhibitor (iAhR) but unaffected by ALDH1A3 or FSP1 inhibitors. FSP1 levels remained un- changed. (e–g) Quantification of oxidative stress markers. IDA reduced 4-HNE and MDA levels and increased GSH levels, effects negated by iAhR, iALDH1A3, or iFSP1. Data are mean ± SEM (n = 3). One-way ANOVA with Tukey's post hoc test for (d); unpaired t-test for (b, c, e–g). p values were adjusted for multiple testing by the Benjamini Hochberg method. **p < 0.01, ***p < 0.001 vs. control; ##p < 0.01, ###p < 0.001 vs. IDA group; ns (above bars), p > 0.05 vs. ONC + DHF; ns (above horizontal lines), p > 0.05 among groups below the line.

Article Snippet: IDA's impact on ferroptosis proteins and AhR, ALDH1A3, and FSP1 inhibitors was examined in vitro and in vivo using rabbit anti- AhR (1:1000, #SAB4500725, Sigma), rabbit anti- ALDH1A3 (1:1000, #ABN427, Sigma), rabbit anti- FSP1 (1:1000, #A06541- 2, Boster), rabbit anti- GPX4 (1:1000, #SAB5700944, Sigma), rabbit anti- ACSL4 (1:2000, #SAB2100035, Sigma), rabbit anti- SLC7A11 (1:1000, #SAB5700735, Sigma), rabbit anti- GCH1 (1:1000, #PA5- 103865, Invitrogen), rabbit anti- FTH1 (1:500, #ZRB2695, Sigma), rabbit anti- DHODH (1:1000, #SAB2100574, Sigma), rabbit anti- 4- HNE (1:1000, #MA5- 27570, Invitrogen), and rabbit anti- β- actin (1:2000, #AF5003, Beyotime).

Techniques: Western Blot, Control

Journal: CNS neuroscience & therapeutics

Article Title: Oral 7,8-Dihydroxyflavone Protects Retinal Ganglion Cells by Modulating the Gut-Retina Axis and Inhibiting Ferroptosis via the Indoleacrylic Acid-AhR-ALDH1A3-FSP1 Pathway.

doi: 10.1111/cns.70442

Figure Lengend Snippet: FIGURE 6 | Neuroprotective effects of 7,8-DHF and the gut microbiota-IDA-AhR-ALDH1A3-FSP1 pathway on RGC survival and retinal function following ONC injury. (a) Retinal flat-mount images of RBPMS-labeled RGCs. Scale bar: 100 μm. (b) Representative PhNR traces showing retinal function. (c) Quantification of RGC survival (%). RGC density was reduced in the ONC group compared to the Sham group, partially preserved by 7,8-DHF, attenuated by antibiotics, and restored by IDA. AhR, ALDH1A3, and FSP1 inhibitors partially reduced RGC survival. (d) Quantification of PhNR amplitudes (μV). Retinal function decreased in the ONC group, was partially restored by 7,8-DHF, diminished by antibiotics, and recov- ered by IDA. AhR, ALDH1A3, and FSP1 inhibitors partially reduced the recovery. Data are mean ± SD (n = 6). Unpaired t-test was used except for Sham vs. ONC in (c), where Welch's t-test was applied; +++p < 0.001 vs. Sham; *p < 0.05, ***p < 0.001 vs. ONC; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. ONC + DHF; &&p < 0.01, &&&p < 0.001 vs. ONC + DHF + Abx.

Article Snippet: IDA's impact on ferroptosis proteins and AhR, ALDH1A3, and FSP1 inhibitors was examined in vitro and in vivo using rabbit anti- AhR (1:1000, #SAB4500725, Sigma), rabbit anti- ALDH1A3 (1:1000, #ABN427, Sigma), rabbit anti- FSP1 (1:1000, #A06541- 2, Boster), rabbit anti- GPX4 (1:1000, #SAB5700944, Sigma), rabbit anti- ACSL4 (1:2000, #SAB2100035, Sigma), rabbit anti- SLC7A11 (1:1000, #SAB5700735, Sigma), rabbit anti- GCH1 (1:1000, #PA5- 103865, Invitrogen), rabbit anti- FTH1 (1:500, #ZRB2695, Sigma), rabbit anti- DHODH (1:1000, #SAB2100574, Sigma), rabbit anti- 4- HNE (1:1000, #MA5- 27570, Invitrogen), and rabbit anti- β- actin (1:2000, #AF5003, Beyotime).

Techniques: Labeling

Journal: CNS neuroscience & therapeutics

Article Title: Oral 7,8-Dihydroxyflavone Protects Retinal Ganglion Cells by Modulating the Gut-Retina Axis and Inhibiting Ferroptosis via the Indoleacrylic Acid-AhR-ALDH1A3-FSP1 Pathway.

doi: 10.1111/cns.70442

Figure Lengend Snippet: FIGURE 7 | Effects of 7,8-DHF, gut microbiota disruption, and IDA on AhR-ALDH1A3-FSP1 pathway proteins and oxidative stress in retinal tissues following ONC injury. (a) Representative western blot images for AhR-ALDH1A3-FSP1 pathway proteins. (b–d) Quantification of nAhR, ALDH1A3, and FSP1 levels relative to β-actin. 7,8-DHF increased nAhR and ALDH1A3 levels, which were reduced by antibiotics and restored by IDA. AhR inhibitors decreased nAhR and ALDH1A3, while ALDH1A3 or FSP1 inhibition had no effect on nAhR. FSP1 levels remained unchanged across groups. (e–g) Oxidative stress markers: 7,8-DHF reduced 4-HNE and MDA while increasing GSH levels, effects reversed by antibiotics and restored by IDA. Inhibitors of AhR, ALDH1A3, and FSP1 partially reversed these effects, implicating the AhR-ALDH1A3-FSP1 pathway. Data are mean ± SEM (n = 3). p values were adjusted for multiple testing by the Benjamini Hochberg method. *p < 0.05, **p < 0.01 vs. ONC; #p < 0.05 vs. ONC + DHF; &p < 0.05, &&p < 0.01 vs. ONC + DHF + Abx; ns (above bars), p > 0.05 vs. ONC + DHF; ns (above horizontal lines), p > 0.05 among groups below the line.

Article Snippet: IDA's impact on ferroptosis proteins and AhR, ALDH1A3, and FSP1 inhibitors was examined in vitro and in vivo using rabbit anti- AhR (1:1000, #SAB4500725, Sigma), rabbit anti- ALDH1A3 (1:1000, #ABN427, Sigma), rabbit anti- FSP1 (1:1000, #A06541- 2, Boster), rabbit anti- GPX4 (1:1000, #SAB5700944, Sigma), rabbit anti- ACSL4 (1:2000, #SAB2100035, Sigma), rabbit anti- SLC7A11 (1:1000, #SAB5700735, Sigma), rabbit anti- GCH1 (1:1000, #PA5- 103865, Invitrogen), rabbit anti- FTH1 (1:500, #ZRB2695, Sigma), rabbit anti- DHODH (1:1000, #SAB2100574, Sigma), rabbit anti- 4- HNE (1:1000, #MA5- 27570, Invitrogen), and rabbit anti- β- actin (1:2000, #AF5003, Beyotime).

Techniques: Disruption, Western Blot, Inhibition

Journal: Cancer cell

Article Title: Anti-PD-1 immunotherapy with androgen deprivation therapy induces robust immune infiltration in metastatic castration-sensitive prostate cancer

doi: 10.1016/j.ccell.2023.10.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-LAG-3 , BioSB , Cat#BSB3366.

Techniques: Sequencing, Multiplex Assay, Software